Document Type : Research Paper

Authors

1 Ph.D. student, Department of Chemical Engineering, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

2 Assistant Professor / Department of Chemical Engineering, Faculty of Engineering, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

3 Associate Professor, Department of Chemical Engineering, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

Abstract

Antibiotics are used as the main medicine for infectious diseases. However, the usage of antibiotics is always associated with two major problems: side effects and drug resistance. Herbal compounds have been used as alternative medicines for a long time since they have fewer side effects than other medicines. Since different species of Echinops dichorus L. have demonstrated antibacterial effects, this study has used Disk Diffusion Method (DDM) to investigate the antimicrobial effects of ethanolic and aqueous extracts of Echinops dichorus L. and determined the minimum inhibition on a 24-hour culture of the standard Escherichia coli strain. After drying and confirmation, Echinops dichorus L. was collected, and ethanolic and aqueous extracts were prepared using the maceration method. The fractions were kept in clean containers and under standard conditions until they were used after concentrating and drying. Ethanolic and aqueous extracts were able to show an antimicrobial effect against Escherichia coli at MIC = 31.25 µg /ml (12 mm diameter of halo growth) and MIC = 125 μg (10 mm diameter of halo growth), respectively. The antibacterial effects of aqueous and ethanolic extracts of Echinops dichorus L. were found to be acceptable in this study.

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