Document Type : Research Paper


1 Assist. Professor, Department of Soil Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran

2 Professor, Department of Soil Science, Department of Agriculture, Shahrekord University, Shahrekord, Iran


Zinc is one of the essential micronutrients for plant growth and its deficit is often reported in calcareous soils. The total amount of soil Zn does not necessarily indicate Zn availability for plant. Therefore, finding an appropriate extractant, in order to estimate plant available Zn in soil is important. Considering that little information in Iran is available regarding available Zn, in heavy metals contaminated calcareous soils, therefore, assessment of status of available Zn for plants by chemical extractants is very important and necessary. The aim of this research was to assess extraction methods Mehlich 3, AB-DTPA and DTPA-TEA to estimate the sunflower available Zn in 9 contaminated soils. Sunflower plant parameters including dry matter, concentration, and Zn uptake were determined in a pot experiment. The results showed that in contaminated soils, the highest concentration of Zn by use of AB-DTPA and the lowest concentration of Zn by use of DTPA-TEA were extracted. Moreover, in contaminated soils significant correlation wasfound between extracted Zn by DTPA-TEA with concentration and Zn uptake (r=0.78). There were significant correlations between extracted Zn by Mehlich 3 with concentration (r=0.68) and Zn uptake by plant (r=0.80). Thus, it can be concluded that DTPA-TEA and Mehlich 3 methodes can be used to estimate the ability of sunflower-available Zn in contaminated soils.


Main Subjects

Alvarez J. M., Lopez - Valdivia L. M., Novillo J., Obrador A. and Rico M. I. (2006). Comparison of EDTA and sequential extraction tests for phytoavailability prediction of manganese and zinc in agricultural alkaline soils. Geoderma, 132, 450- 463. 
Campbell C. R. and Plank C. O. (1998). Preparation of plant tissue for laboratory analysis. p. 37-50. In Kalra Y. P. (ed), Handbook of Reference Methods for Plant Analysis. CRC Press, Taylor and Francis Group.
Chaignon V., Sanchez - Neira I., Herrmann P., Jaillard B. and Hinsinger P. (2003). Copper bioavailability and extractability as related to chemical properties of contaminated soils from a vine-growing area. Environ. Pollut., 123, 229–238. 
Cu S., Zhou Q. and Chao L. (2007). Potential hyperaccumulation of Pb, Zn, Cu and Cd in Endurant plants distributed in old smeltry, Northeast China. Environ. Geol., 51, 1043-1048.
Feng M. H., Shan X. Q., Zhang S. Z. and Wen B. (2005). Comparison of a rhizosphere-based method with other one step extraction methods for assessing the bioavailability of soil metals to wheat. Chemosphere, 59, 939-949.  
Gupta A.  K. and Sinha S. (2006). Chemical fractionation and heavy metals accumulation in the plants of (Sesamum indicum L.) var. T55 grown on soil amended with tannery sludge: selection of single extractants. Chemosphere, 64, 161– 173. 
Lasat M. (2002). Phytoextraction of metal from contaminated soil. A review of plant soil metal interaction and assessment of pertinent agronomic issues. J. Hazard. Mater., 3, 1-25. 
Lindsay W.  L. (1979). Chemical equilibria in soils. John Wiley and sons, New York. 
Lindsay W. L. and Norvell W. A. (1978).  Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci. Soc. Am. J., 42, 421-428.
Loeppert R. H. and Suarez D. L. (1996). Carbonate and gypsum. p. 437-474. In Sparks D. L. (ed), Methods of Soil Analysis. SSSA, Madison.
Malakouti M. J. (2007). Zinc is a neglected element in the life cycle of plants. Middle E. Russ. J. Plant Sci. Biotechnol., 1(1), 1-12.
Mehlich A. (1984). Mehlich 3 soil test extractant: A modification of Mehlich 2 extractant. Commun. Soil Sci. Plant Anal., 15, 1409-1416. 
Motaghian H. R. and Hosseinpour A. (2013). Assessment of several extractants for the determination of zinc bioavailability to Bean (Phaseolus vulgaris L.) in calcareous soils amended and unamended with sewage sludge. J. Water and Soil., 27(4), 742-752 [In Persian]. 
Motaghian H. R., Hosseinpour A., Raeisie F. and Mohamadi J. (2017). Assessment of several extractants for the determination of zinc bioavailability to Wheat (Triticum aestivum L.) in calcareous soils amended and unamended with sewage sludge. J. Water and Soil Sci (Sci. and Technol. Agric. and Natur. Resour.)., 1, 13-20 [In Persian]. 
Nelson D. W. and Sommers L. E. (1996). Carbon, organic carbon, and organic matter. p. 961-1010. In Sparks D. L. (ed), Methods of Soil Analysis. SSSA, Madison. 
Saffari M., Yasrebi J., Karimian N. and Shan X. Q. (2009). Evaluation of three sequential extraction methods for fractionation of zinc in calcareous and acidic soils. Research J. Biol. Sci., 4, 848-857. 
Gee G. W. and Bauder J. W. (1986). Particle size analysis. p. 404-407. In Klute A. (ed), Methods of Soil Analysis. Part 1. 2nd edition. Agron. Monogr. 9. ASA and SSSA, Madison, WI.
Haddad K. S. and Evans J. C. (1993). Assessment of chemical methods for extracting zinc, manganese, copper, and iron from New South Wales Soils. Commun. Soil Sci. Plant Anal., 24, 29-44.
Malakouti M. J., Keshavarz P. and Karimian N. A. (2009). Comprehensive diagnosis and optimal fertilizer recommendation for sustainable agriculture. Tarbiat Modares University Press, Tehran. 755 pp [In Persian]. 
Sakal R., Singh A. P., Sinha H. and Thakur K. N. (1981). Evaluation of critical concentration of zinc in rice and wheat grown in Tarai soils. J. of Indian Soc. S. Sci., 29, 107-109. 
Sahuquillo A., Rigol A. and Rauret G. (2003). Overview of the use of leaching/extraction tests for risk assessment of trace metals in contaminated soils and sediments. Trend Anal. Chem., 22, 152-159. 
Singh C. P., Prasad R. N., Sinba H. and Kanke B. (1977). Evaluation of the critical limit and extractants for the determination of available zinc in calcareous soils. Beitrage trop. Landwirtsch Veterinärmed., 15, 131136. 
Soltanpour P. N. and Schwab A. P. (1977).  A new soil test for simultaneous extraction of macro- and micro-nutrients in alkaline soils. Commun. Soil Sci. Plant Anal., 8(3), 195207. 
 Sposito G. L., Lund J. and Chang A. C. (1982). Trace metal chemistry in arid-zone field soils amended with sewage sludge: I. Fractionation of Ni, Cu, Zn, Cd, and Pb in solid phases. Soil Sci. Soc. Am. J., 46, 260265. 
Sumner M. E. and Miller P. M. (1996). Cation exchange capacity and exchange coefficient. p. 1201-1230. In Sparks D. L. (ed), Methods of Soil Analysis. SSSA, Madison. 
Tembo B. D., Sichilongo K. and Cernak J. (2006). Distribution of copper, lead, cadmium and zinc concentrations in soils around Kabwe town in Zambia. Chemosphere, 63, 497–501.